Current Development in Bioanalytical Sample Preparation Techniques
DOI:
https://doi.org/10.47419/bjbabs.v4i04.226Keywords:
Bioanalysis, Sample preparation, Microextraction, Biological sample matrices.Abstract
The preparation of the sample is the most important stage in bioanalysis. Proteins, salts, and other organic compounds with chemical characteristics similar to the target analytes are commonly found in biological samples. As a result, sample preparation is an essential step that improves matrix suitability for analysis in multiple ways, including by separating the analytes and clearing the matrix of obstructive elements. Innovative sample preparation techniques have been more and more popular over the last 10 years due to their advantages over conventional techniques in terms of accuracy, automation, simplicity of sample preparation, storage, and delivery. This article’s goal is to raise awareness of the most recent advancements in the processing of bioanalytical samples. Different extraction stages are provided by modern techniques, such as sorbent-based microextraction, and the advantages of bioanalytical approaches have been highlighted.
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Moein MM, Beqqali AE, Abdel-Rehim, M. Bioanalytical method development and validation: Critical concepts and strategies. J of Chromatography B. 2017;1043:3– DOI: https://doi.org/10.1016/j.jchromb.2016.09.028
27720355. Available from: https://pubmed.ncbi.nlm.nih.gov/27720355/. 10.1016/j.jchromb.2016.09.028.
Ashri NY, Abdel-Rehim, M. Sample treatment based on extraction techniques in biological matrices. Bioanalysis. 2011;3:2003–2018. 21899508. Available from: https: DOI: https://doi.org/10.4155/bio.11.201
//pubmed.ncbi.nlm.nih.gov/21899508/. 10.4155/bio.11.201.
Vaghela A, Patel A. Sample preparation in bioanalysis: A review. INTERNATIONAL JOURNAL OF SCIENTIFIC & TECHNOLOGY RESEARCH. 2016;5(5):6–10. Available from: https://www.ijstr.org/final-print/may2016/Sample-Preparation-In- Bioanalysis-A-Review.pdf.
Kohler I, Guillarme D. Multi-target screening of biological samples using LC-MS/MS: focus on chromatographic innovations. Bioanalysis. 2014;6:1255– 1273. 24946925. Available from: https://pubmed.ncbi.nlm.nih.gov/24946925/. 10.4155/bio.14.80. DOI: https://doi.org/10.4155/bio.14.80
Lopes BR, Barreiro JC, Cass QB. Bioanalytical challenge: A review of environ- mental and pharmaceuticals contaminants in human milk. J Pharm Biomed Anal. 2016;130:318–325. 27372148. Available from: https://pubmed.ncbi.nlm.nih.gov/ 27372148/. 10.1016/j.jpba.2016.06.012. DOI: https://doi.org/10.1016/j.jpba.2016.06.012
Buszewski B, Szultka M. Past present and future of solid phase extraction: A review. Crit Rev Anal Chem. 2012;42:198–213. DOI: https://doi.org/10.1080/07373937.2011.645413
Alexovic M, Dotsikas Y, Bober P. Achievements in robotic automation of solvent extraction and related approaches for bioanalysis of pharmaceuti- cals. J of Chromatography B Analyt Technol Biomed Life Sci. 2018;1092:402– DOI: https://doi.org/10.1016/j.jchromb.2018.06.037
29945105. Available from: https://pubmed.ncbi.nlm.nih.gov/29945105/. 10.1016/j.jchromb.2018.06.037.
Arthur CL, Pawliszyn J. Solid-phase microextraction with thermal desorption using fused silica optical fiber. Anal Chem. 1990;62:2145–2148. Available from: https://pubs.acs.org/doi/10.1021/ac00218a019. DOI: https://doi.org/10.1021/ac00218a019
Boyaci E, Rodríguez LA, Gorynski K. Sample preparation with solid phase microex- traction and exhaustive extraction approaches: comparison for challenging cases. Anal Chim Acta. 2015;873:14–30. 25911426. Available from: https://pubmed.ncbi. nlm.nih.gov/25911426/. 10.1016/j.aca.2014.12.051. DOI: https://doi.org/10.1016/j.aca.2014.12.051
Galievsky V, Pawliszyn J. Fluorometer for screening of doxorubicin in perfusate solu- tion and tissue with solid-phase microextraction chemical biopsy sampling. Anal Chem. 2020;92:13025–13033. 15039931. Available from: https://pubmed.ncbi.nlm. nih.gov/15039931/. 10.1002/jms.606. DOI: https://doi.org/10.1021/acs.analchem.0c01905
Liu H, Dasgupta PK. Analytical chemistry in a drop solvent extraction in a micro- drop. Anal Chem. 1996;68:1817–1821. 32847350. Available from: https://pubmed. ncbi.nlm.nih.gov/32847350/. 10.1021/acs.analchem.0c01905. DOI: https://doi.org/10.1021/ac960145h
Liu H, Dasgupta PK. Analytical chemistry in a drop. Solvent extraction in a micro- drop. NIH NLM;21619093. Available from: https://pubmed.ncbi.nlm.nih.gov/ 21619093/. 10.1021/ac960145h.
Filippou O, Bitas D, Samanidou V. Green approaches in sample preparation of bioanalytical samples prior to chromatographic analysis. J Chromatogr B Ana- lyt Technol Biomed Life Sci. 2017;1043:44–62. 27623110. Available from: https: DOI: https://doi.org/10.1016/j.jchromb.2016.08.040
//pubmed.ncbi.nlm.nih.gov/27623110/. 0.1016/j.jchromb.2016.08.040.
Jeannot MA, Cantwell FF. Solvent microextraction into a single drop. Anal Chem. 1996;68:2236–2240. 21619310. Available from: https://pubmed.ncbi.nlm.nih.gov/ 21619310/. 10.1021/ac960042z. DOI: https://doi.org/10.1021/ac960042z
Ochiai N, Sasamoto K, David F. Solvent-assisted stir bar sorptive extraction by using swollen polydimethylsiloxane for enhanced recovery of polar solutes in aque- ous samples: application to aroma compounds in beer and pesticides in wine. J Chromatogr A. 2016;1455:45–56. 27289502. Available from: https://pubmed.ncbi. nlm.nih.gov/27289502/. 10.1016/j.chroma.2016.05.085. DOI: https://doi.org/10.1016/j.chroma.2016.05.085
He M, Wang Y, Zhang Q. Stir bar sorptive extraction and its application. J Chro- matogr A. 2021;1637:461810–461810. DOI: https://doi.org/10.1016/j.chroma.2020.461810
Clark KD, Zhang C, Anderson JL. Sample preparation for bioanalytical and phar- maceutical analysis. Anal Chem. 2016;88:11262–11270. 27779849. Available from: https://pubmed.ncbi.nlm.nih.gov/27779849/. 10.1021/acs.analchem.6b02935. DOI: https://doi.org/10.1021/acs.analchem.6b02935
Campillo N, Vinas P, Sandrejova J, Andruch V. Ten years of dispersive liquid- liquid microextraction and derived techniques. Appl Spectrosc Rev. 2017;52:267– 415. Available from: https://colab.ws/articles/10.1080/05704928.2016.1224240. 10.1080/05704928.2016.1224240. DOI: https://doi.org/10.1080/05704928.2016.1224240
Yilmaz E, Soylak M. Latest trends green aspects and innovations in liquid-phase- based microextraction techniques: A review. Turkish J Chem. 2016;40:868–893. Available from: https://journals.tubitak.gov.tr/chem/vol40/iss6/2/. 10.3906/kim- 1605-26. DOI: https://doi.org/10.3906/kim-1605-26
Diuzheva A, Carradori S, Andruch V, Locatelli M, De LE, Tiecco M, et al. Use of innovative (micro)extraction techniques to characterize harpagophytum procum-bens root and its commercial food supplements. Phytochem Anal. 2018;29(3):233– 241. DOI: https://doi.org/10.1002/pca.2737
Mansour FR, Danielson ND. Solidification of floating organic droplet in dispersive liquid liquid microextraction as a green analytical tool. Talanta. 2017;170:22–35. Available from: https://www.sciencedirect.com/science/article/ abs/pii/S0039914017303818. https://doi.org/10.1016/j.talanta.2017.03.084. DOI: https://doi.org/10.1016/j.talanta.2017.03.084
Zhao Y, Hou X, Qin D. Dispersive liquid-liquid microextraction method for the simultaneous determination of four isomers of hexa-chlorocyclohexane and six pyrethroid pesticides in milk by gas chromatography electron capture detector. Food Anal Methods. 2020;13:370–381. 10.1007/s12161-019-01662-w. DOI: https://doi.org/10.1007/s12161-019-01662-w
Asati A, Satyanarayana G, Patel DK. Comparison of two microextraction methods based on solidification of floating organic droplet for the determination of multiclass analytes in river water samples by liquid chromatography tandem mass spectrometry using Central Composite Design. J Chromatogr A. 2017;1513:157–171. DOI: https://doi.org/10.1016/j.chroma.2017.07.048
Ferrone V, Cotellese R, Carlucci M. Air assisted dispersive liquid-liquid microextraction with solidification of the floating organic droplets (AA-DLLME- SFO) and UHPLC-PDA method: application to antibiotics analysis in human plasma of hospital acquired pneumonia patients. J Pharm Biomed Anal. 2018;151:266–273. Available from: https://www.sciencedirect.com/science/article/ abs/pii/S0731708517319830. https://doi.org/10.1016/j.jpba.2017.12.039. DOI: https://doi.org/10.1016/j.jpba.2017.12.039
Kiarostami V, Rouini MR, Mohammadian R. Binary solvents dispersive liquid- liquid microextraction (BS-DLLME) method for determination of tramadol in urine using high-performance liquid chromatography. DARU J Pharm Sci. 2014;22:25– DOI: https://doi.org/10.1186/2008-2231-22-25
24495475. Available from: https://pubmed.ncbi.nlm.nih.gov/24495475/. 10.1186/2008-2231-22-25.
Pedersen BS, Rasmussen KE. Recovery, enrichment and selectivity in liquid-phase microextraction-comparison with conventional liquid-liquid extraction. J Chro- matogr A. 2002;963(1-2):3–17. 12187984. Available from: https://pubmed.ncbi. nlm.nih.gov/12187984/. 10.1016/s0021-9673(02)00215-7. DOI: https://doi.org/10.1016/S0021-9673(02)00215-7
Pedersen BS, Rasmussen KE. Electrokinetic migration across artificial liquid mem- branes - new concept for rapid sample preparation of biological fluids. J Chromatogr A. 2006;1109(2):183–190. 16445928. Available from: https://pubmed.ncbi.nlm.nih. gov/16445928/. 10.1016/j.chroma.2006.01.025. DOI: https://doi.org/10.1016/j.chroma.2006.01.025
Morales C, Cardenas S, Simonet BM, Valcarcel M. Sample treatments improved by electric fields. Analyt Trend Anal Chem. 2010;29(2):158–165. DOI: https://doi.org/10.1016/j.trac.2009.11.006
Kuban P, Slampova A, Bocek P. Electric field-enhanced transport across phase boundaries and membranes and its potential use in sample pretreatment for bio- analysis. Electrophoresis. 2010;31(5):768–785. 20191542. Available from: https: DOI: https://doi.org/10.1002/elps.200900561
//pubmed.ncbi.nlm.nih.gov/20191542/. 10.1002/elps.200900561.
Yan J, Kuzhiumparambil U, Bandodkar S. Development and validation of a simple rapid and sensitive LC-MS/MS method for the measurement of urinary neurotrans- mitters and their metabolites. Anal Bioanal Chem. 2017;409:7191–7199. 29030665. DOI: https://doi.org/10.1007/s00216-017-0681-3
Available from: https://pubmed.ncbi.nlm.nih.gov/29030665/. 10.1007/s00216-017- 0681-3.
Yu X, Li X, You S. Electromembrane extraction of chlorprothixene, haloperidol and risperidone from whole blood and urine. J Chromatogr A. 2020;1629:461480– 461480. 32827905. Available from: https://pubmed.ncbi.nlm.nih.gov/32827905/. 10.1016/j.chroma.2020.461480. DOI: https://doi.org/10.1016/j.chroma.2020.461480
Venson R, Korb AS, Cooper G. A review of the application of hollow-fiber liquid-phase microextraction in bioanalytical methods-a systematic approach with focus on forensic toxicology. J Chromatogr B Analyt Technol Biomed Life Sci. 2019;1108:32–53. 30682538. Available from: https://pubmed.ncbi.nlm.nih.gov/ 30682538/. 10.1016/j.jchromb.2019.01.006. DOI: https://doi.org/10.1016/j.jchromb.2019.01.006
Shukri D, Sanagi MM, Ibrahim W. Liquid chromatographic determination of NSAIDs in urine after dispersive liquid-liquid microextraction based on solidification of floating organic droplets. Chromatographia. 2015;78:987–994. 10.1007/s10337-015-2920-0. DOI: https://doi.org/10.1007/s10337-015-2920-0
Arain SS, Kazi TG, Arain JB. Preconcentration of toxic elements in artificial saliva extract of different smokeless tobacco products by dual-cloud point extraction. Microchem J. 2014;112:42–49. 23566880. Available from: https://pubmed.ncbi.nlm. nih.gov/23566880/. 10.1016/j.ecoenv.2013.03.001. DOI: https://doi.org/10.1016/j.microc.2013.09.005
Li N, Zhang T, Chen G. Recent advances in sample preparation techniques for quan- titative detection of pharmaceuticals in biological samples. TrAC Trends Anal Chem. 2021;142:116318–116318. DOI: https://doi.org/10.1016/j.trac.2021.116318
Olcer YA, Tascon M, Eroglu AE. Thin film microextraction: towards faster and more sensitive microextraction. TrAC Trends Anal Chem. 2019;113:93–101. Available from: https://open.metu.edu.tr/handle/11511/48426. https://doi.org/10.1016/j.trac.2019.01.022. DOI: https://doi.org/10.1016/j.trac.2019.01.022
El-Sherbeni AA, Stocco MR, Wadji FB. Addressing the instability issue of dopamine during microdialysis: the determination of dopamine serotonin methamphetamine and its metabolites in rat brain. J Chromarogr A. 2020;1627:461403–461403. Avail- able from: https://pmc.ncbi.nlm.nih.gov/articles/PMC7484461/. DOI: https://doi.org/10.1016/j.chroma.2020.461403
Chepyala D, Kuo HC, Su KY. Improved dried blood spot-based metabolomics analysis by a postcolumn infused-internal standard assisted liquid chromatography- electrospray ionization mass spectrometry method. Anal Chem. 2019;91:10702– 10712. Available from: https://pubmed.ncbi.nlm.nih.gov/31361473/. DOI: https://doi.org/10.1021/acs.analchem.9b02050
Nys G, Kok M, Servais AC. Beyond dried blood spot: current microsampling techniques in the context of biomedical applications. TrAC Trends Anal Chem. 2017;97:326–332. 10.1016/j.trac.2017.10.002. DOI: https://doi.org/10.1016/j.trac.2017.10.002
Capiau S, Veenhof H, Koster RA. Official International Association for Therapeutic Drug Monitoring and Clinical Toxicology Guideline: development and validation of dried blood spot-based methods for therapeutic drug monitoring. Ther Drug Monit. 2019;41:409–430. 31268966. Available from: https://pubmed.ncbi.nlm.nih. gov/31268966/. 10.1097/FTD.0000000000000643. DOI: https://doi.org/10.1097/FTD.0000000000000643
Lombardi V, Carassiti D, Giovannoni G. The potential of neurofilaments analysis using dry-blood and plasma spots. Sci Rep. 2020;10:97–97. PMC6952412. Available from: https://pubmed.ncbi.nlm.nih.gov/31919375/. 10.1038/s41598-019-54310-y. DOI: https://doi.org/10.1038/s41598-019-54310-y
Parker SL, Lipman J, Dimopoulos G. A validated method for the quantifi- cation of fosfomycin on dried plasma spots by HPLC-MS/MS: application to a pilot pharmacokinetic study in humans. J Pharm Biomed Anal. 2020;115:509– DOI: https://doi.org/10.1016/j.jpba.2015.07.013
26299527. Available from: https://pubmed.ncbi.nlm.nih.gov/26299527/. 10.1016/j.jpba.2015.07.013.
Li W, Doherty J, Favara S. Evaluation of plasma microsampling for dried plasma spots (DPS) in quantitative LC-MS/MS bioanalysis using ritonavir as a model compound. J Chromatogr B Anal Technol Biomed Life Sci. 2015;991:46–52. 25913423. Available from: https://pubmed.ncbi.nlm.nih.gov/25913423/. 10.1016/j.jchromb.2015.03.026. DOI: https://doi.org/10.1016/j.jchromb.2015.03.026
Gonzalez D, Melloni C, Poindexter BB. Simultaneous determination of trimetho- prim and sulfamethoxazole in dried plasma and urine spots. Bioanalysis. 2015;7:1137–1149. 23566880. Available from: https://pubmed.ncbi.nlm.nih.gov/ 26039810/. 10.1016/j.ecoenv.2013.03.001. DOI: https://doi.org/10.4155/bio.15.38
Meesters R, Hooff G, Van Huizen N. Impact of internal standard addition on dried blood spot analysis in bioanalytical method development. Bioanalysis. 2011;3:2357– 2364. 22011183. Available from: https://pubmed.ncbi.nlm.nih.gov/22011183/. 10.4155/bio.11.202. DOI: https://doi.org/10.4155/bio.11.202
Zheng N, Zeng J, Ji QC. Bioanalysis of dried saliva spot (DSS) samples using detergent-assisted sample extraction with UHPLC-MS/MS detection. Anal Chim Acta. 2016;934:170–179. 27506357. Available from: https://pubmed.ncbi.nlm.nih. gov/27506357/. 10.1016/j.aca.2016.05.057. DOI: https://doi.org/10.1016/j.aca.2016.05.057
Hsiao YC, Lin SY, Chien K. An immuno-MALDI mass spectrometry assay for the oral cancer biomarker, matrix metalloproteinase-1, in dried saliva spot samples. Anal Chim Acta. 2020;1100:118–130. 31987131. Available from: https://pubmed.ncbi. nlm.nih.gov/31987131/. 10.1016/j.aca.2019.12.006. DOI: https://doi.org/10.1016/j.aca.2019.12.006
Numako M, Toyo'oka T, Noge I. Risk assessment of diabetes mellitus using dried saliva spot followed by ultra-performance liquid chromatography with flu- orescence and mass spectrometry. Microchem J. 2018;142:202–207. Available from: https://www.sciencedirect.com/science/article/abs/pii/S0026265X18304107. https://doi.org/10.1016/j.microc.2018.06.037. DOI: https://doi.org/10.1016/j.microc.2018.06.037
Pablo A, Breaud AR, Clarke W. Automated analysis of dried urine spot (DUS) samples for toxicology screening. Clin Biochem. 2020;75:70–77. 31707014. Available from: https://pubmed.ncbi.nlm.nih.gov/31707014/. 10.1016/j.clinbiochem.2019.10.009. DOI: https://doi.org/10.1016/j.clinbiochem.2019.10.009
Dhahouri A, Langhans N, D C, Hammadi A, Z. Quantification of methylci- trate in dried urine spots by liquid chromatography tandem mass spectrometry for the diagnosis of propionic and methylmalonic acidemias. Clin Chim Acta. 2018;487:41–45. 30217751. Available from: https://pubmed.ncbi.nlm.nih.gov/ 30217751/. 10.1016/j.cca.2018.09.017. DOI: https://doi.org/10.1016/j.cca.2018.09.017
Kok M, Fillet M. Volumetric absorptive microsampling: current advances and appli- cations. J Pharm Biomed Anal. 2018;147:288–296. 28803682. Available from: https://pubmed.ncbi.nlm.nih.gov/28803682/. 10.1016/j.jpba.2017.07.029. DOI: https://doi.org/10.1016/j.jpba.2017.07.029
Iskierko Z, Sharma PS, Prochowicz D, Fronc K, Souza D, Toczydłowska F, et al. Molecularly Imprinted Polymer (MIP) Film with Improved Surface Area Devel- oped by Using Metal-Organic Framework (MOF) for Sensitive Lipocalin (NGAL) Determination. ACS Appl Mater Interfaces. 2016;8:19860–19865. Available from: https://pubs.acs.org/doi/abs/10.1021/acsami.6b05515. DOI: https://doi.org/10.1021/acsami.6b05515
Qian K, Fang G, Wang S. A novel core-shell molecularly imprinted polymer based on metal-organic frameworks as a matrix. Chem Commun. 2011;47:10118–10120. Available from: https://pubs.rsc.org/en/content/articlelanding/2011/cc/c1cc12935j DOI: https://doi.org/10.1039/c1cc12935j
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