Chemical profiling and antimicrobial potential of crude extract and essential oil from Conyza bonariensis leaves
DOI:
https://doi.org/10.47419/bjbabs.v4i03.244Keywords:
Antimicrobial activity, Antimicrobial Resistance, Conyza bonariensis, Essential OilAbstract
Microbial infections have made significant contributions to the global health burden, particularly in developing countries with lagging economies. Challenges arising from the evolution of antimicrobial-resistant (AMR) bacteria have led to the search for novel antimicrobial agents, especially those derived from medicinal plants. Therefore, this study aimed to assess the antibacterial activity of ethyl acetate crude extracts and essential oils from C. bonariensis collected from the Mbeya region of Tanzania. Initial experimental activities involved the preparation of crude extracts and essential oil, which were then tested for antimicrobial activity against selected test organisms (Staphlococcus aureus (ATCC29213), Bacillus subtilis (ATCC6051), Escherichia coli (ATCC8736), Salmonella typhi (ATCC6539), and Candida albicans (DSM1665)) by using the disc diffusion method. On the other hand, the chemical composition profiling of ethyl acetate and essential oils was performed by gas chromatography mass spectrometry (GC-MS). The crude extracts and essential oil of C. bonariensis leaves from Tanzania were demonstrated antibacterial and antifungal activity. The minimum inhibitory concentration (MIC) of leaves' ethyl acetate crude extract was 25 mg/mL > MIC > 12.5 mg/mL for tested organisms, with the exception of C. albicans, where the MIC was 50 mg/mL > MIC > 25 mg/mL. Similarly, for all tested organisms, the MIC of leaves essential oils was :1 > MIC > l:2, except S. typhi, where the MIC was 2:1 > MIC > 1:1. On the other hand, 2,4-di-tert-butyl phenol in crude leaf extracts had the highest percentage composition of peak area (41%); whereas, in essential of, 2,4-Di-tert-butyl phenol had the highest percentage composition (38.8%). The present findings have demonstrated the antimicrobial potential of ethyl acetate crude extract and essential oil of C. bonariensis leaves from Tanzania and validate the wide use of the plant by local communities, particularly the local community of Mbeya region in Tanzania.
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Blomberg B, Manji KP, Urassa WK, Tamim BS, Mwakagile DS, Jureen R. Antimicrobial resistance predicts death in Tanzanian children with bloodstream infections: a prospective cohort study. BMC Infectious Diseases. 2007;7(43). https://doi.org/10.1186/1471-2334-7-43.
Dadgostar P. Antimicrobial Resistance: Implications and Costs. Infect Drug Resist. 2019;12(3903):10–10. https://doi.org/10.2147/IDR.S234610.
Organization WH. Antimicrobial resistance; 2023. Available from: https://www. who.int/news-room/fact-sheets/detail/antimicrobial-resistance.
Manyahi J, Matee MI, Majigo M, Moyo S, Mshana SE, Lyamuya EF. Predominance of multi-drug resistant bacterial pathogens causing surgical site infections in Muhimbili national hospital. BMC Res Notes. 2014;7(500). https://doi.org/10.1186/1756-0500- 7-500.
Moremi HCN, Stephen E, Mshana. Antimicrobial resistance pattern: a report of microbiological cultures at a tertiary hospital in Tanzania. 2016;https://doi.org/10.1186/s12879-016-2082-1.
Bisi-Johnson MA, Obi CL, Samuel BB, Eloff JN, Okoh AI. Antibacterial activity of crude extracts of some South African medicinal plants against multidrug resis- tant etiological agents of diarrhoea. BMC Complement Altern Med. 2017;17(321). https://doi.org/10.1186/s12906-017-1802-4.
Kianfé BY, Kühlborn J, Tchuenguem RT, Tchegnitegni BT, Ponou BK, Groß
J. Antimicrobial secondary metabolites from the medicinal plant Crinum glaucum A. Chev.(Amaryllidaceae). S Afr J Bot. 2020;(133):161–167. https://doi.org/10.1016/j.sajb.2020.07.026.
Pensantes-Sangay SJ, Calla-Poma RD, Requena-Mendizabal MF, Alvino-Vales MI, Millones-Gómez PA. Chemical Composition and Antibacterial Effect of Plan- tago Major Extract on Periodontal Pathogens 20. Pesqui Bras Odontope- diatria Clín Integr. 2020;Available from: https://doi.org/10.1590/pboci.2020.100. https://doi.org/10.1590/pboci.2020.100.
Urmeela T, Mahomoodally F. Aloe vera extracts: From traditional uses to mod- ern medicine. 2015;p. 211–226. Available from: https://www.researchgate.net/ publication/297766937_Aloe_vera_extracts_From_traditional_uses_to_modern_ medicine.
Masithoh DA, Kusdarwati R, Handijatno D. Antibacterial activity of bitter gourd ( Momordica charantia l .) leaf extract against Aeromonas hydrophila. IOP Conf Ser: Earth Environ Sci. 2019;236:12096–12096. https://doi.org/10.1088/1755- 1315/236/1/012096.
Yang C, U H, Feng DH, Y. Antibacterial activity and mode of action of the Artemisia capillaris essential oil and its constituents against respira- tory tract infection-causing pathogens. Molecular Medicine Reports. 2015;11. https://doi.org/10.3892/mmr.2014.3103.
Hawrelak J, Cattley T, Myers S. Essential Oils in the Treatment of Intestinal Dysbiosis: A Preliminary in vitro Study. Alternative Medicine Review : A Journal of Clinical Therapeutic. 2009;14(380):4–4. Available from: https://pubmed.ncbi.nlm.nih.gov/ 20030464/.
Orchard A, Vuuren SV. Commercial Essential Oils as Potential Antimicrobials to Treat Skin Diseases. Evid Based Complement. Alternat Med. 2017;p. 2017–2017.
Horváth G, S KAC. Essential oils in the treatment of respiratory tract diseases high- lighting their role in bacterial infections and their anti-inflammatory action: Flavour Fragr J. 2015;30(5). https://doi.org/10.1002/ffj.3252.
Ghwanga AAK, Chacha M. Vitro Antimicrobial Activity of Conyza bonariensis and Tribulus terrestris Growing in Tanzania Open. 2019;5(1:4):7–7. 10.21767/2472- 0151.100042.
Abers M, Schroeder S, Goelz L, Sulser A, Rose ST, Puchalski T, et al. Antimicrobial activity of the volatile substances from essential oils BMC Complementary Medicine and Therapies. 2021;21:124–124. https://doi.org/10.1186/s12906-021-03285-3.
Aryal S. McFarland Standards- Principle, Preparation, Uses, Limitations Microbe Notes. 2021;Available from: https://microbenotes.com/mcfarland-standards/.
Reddy DN, Akhtar MS, Swamy MK, Sinniah UR. Essential Oils Extracted from Medicinal Plants and Their Applications. In: editors Natural Bio-active Compounds. 2019;1:237–83. http://dx.doi.org/10.1007/978-981-13-7154-79.
Khaki P, Sharma A, Bhalla P. Comparison of Two Disc Diffusion Methods
with Minimum Inhibitory Concentration for Antimicrobial Susceptibility Testing of Neisseria Gonorrhoeae Isolates. Ann Med Health Sci Res. 2014;4(3):453–459. https://doi.org/10.4103/2141-9248.133477.
Silva ACAD, Matias EFF, Rocha JE, Araújo AC, Freitas TS, Campina FF. Gas chromatography coupled to mass spectrometry (GC-MS) characterization and eval- uation of antibacterial bioactivities of the essential oils from Piper arboreum Aubl Piper aduncum L. e Piper gaudichaudianum Kunth. 2020;76(1-2)(35–42.):35–42. https://doi.org/10.1515/znc-2020-0045.
Viteri R, Peñarreta JP. Antioxidant activity and GC-MS profile of Conyza bonariensis L. leaves extract and fractions. vol. 73 of 3; 2020. https://doi.org/10.15446/rfnam.v73n3.81452.
Chy AMNU, Kamal M, Barlow ATM, Faruque JW, Yang MO, X. Evaluation of anti- nociceptive and anti-inflammatory activities of the methanol extract of Holigarna caustica (Dennst.) Oken leaves. J Ethnopharmacol. 2019;236:401–412. Avail- able from: https://www.researchgate.net/publication/277230263_Antimicrobial_ Effect_of_15_Medicinal_Plant_Species_and_their_Dependency_on_Climatic_ Conditions_of_Growth_in_Different_Geographical_and_Ecological_Areas_of_ Fars_Province.
Klug MJ, Markovetz AJ. Degradation of hydrocarbons by members of the genus Can- dida 3. Oxidative intermediates from 1-hexadecene and 1-heptadecene by Candida lipolytica. J Bacteriol. 1968;96(4):1115–1138. https://doi.org/10.1128/jb.96.4.1115- 1123.1968.
Santos CC, De MP, Salvadori MS, Mota VG, Costa LM, De Almeida AAC, et al. Antinociceptive and Antioxidant Activities of Phytol In Vivo and In Vitro Models. Neurosci J. 2013;949452. https://doi.org/10.1155/2013/949452.
Khan IH, Javaid A. DNA cleavage of the fungal pathogen and production of anti- fungal compounds are the possible mechanisms of action of biocontrol agent Peni- cillium italicum against Macrophomina phaseolina. Mycologia. 2022;114(1):24–34. https://doi.org/10.1080/00275514.2021.1990627.
Gossweiler AG, Martinez-Mier EA. Chapter 6: Vitamins and Oral Health. Monogr Oral Sci. 2020;28:59–67. https://doi.org/10.1159/000455372.
Islam MT, De Alencar MVOB, Machado KDC, Machado KDC, Melo-Cavalcante AADC, De Sousa DP. Phytol in a pharma-medico- stance. Chem Biol Interact. 2015;p. 60–73. https://doi.org/10.1016/j.cbi.2015.07.010.
Kohli V, Sharma D, Sandur SK, Suryavanshi S, Sainis KB. Immune responses to novel allergens and modulation of inflammation by vitamin K3 analogue: a ROS dependent mechanism. Int Immunopharmacol. 2011;11(2):233–276. https://doi.org/10.1016/j.intimp.2010.11.027.
Mandal MD, Mandal S. Honey: its medicinal property and antibacterial activity. Asian Pac J Trop Biomed. 2011;1(2):60016–60022. https://doi.org/10.1016/S2221- 1691(11)60016-6.
Lv JS, Zhang LL, Zhou CXZ, F J. Chemical composition, antioxidant and antimicrobial activity of the extracts of the flowers of the Chinese plant Chimonanthus praecox. Nat Prod Res. 2012;26(14):1363–1370. https://doi.org/10.1080/14786419.2011.602828.
Varsha KK, Devendra L, Shilpa G, Priya S, Pandey, Nampoothiri KM. 4- Di-tert-butyl phenol as the antifungal, antioxidant bioactive purified from a newly isolated Lactococcus sp. Int J Food Microbiol. 2015;15(211):44–50. https://doi.org/10.1016/j.ijfoodmicro.2015.06.025.
Boeck P, Leal PC, Yunes RA, Filho VC, López S, Sortino M. Antifungal activity and studies on mode of action of novel xanthoxyline-derived chalcones. Arch Pharm (Weinheim). 2005;338(2-3):87–95. https://doi.org/10.1002/ardp.200400929.
Veres K, Csupor B, Lázár A, Hohmann J. Antifungal Activity and Composition of Essential Oils of Conyza canadensis Herbs and Roots. Sci World J. 2012;48(46):646– 646. https://doi.org/10.1100/2012/489646.
Skanda S, Vijayakumar BS. Antioxidant and Anti-inflammatory Metabolites of a Soil-Derived Fungus Aspergillus arcoverdensis SSSIHL-01. Curr Microbiol. 2021;78(4):1317–1340. https://doi.org/10.1007/s00284-021-02401-3.
Tonisi S, Okaiyeto K, Hoppe H, Mabinya LV, Nwodo UU, Okoh AI. Chemical con- stituents, antioxidant and cytotoxicity properties of Leonotis leonurus used in the folklore management of neurological disorders in the Eastern Cape. South Africa 3 Biotech. 2020;10(3):141–141. https://doi.org/10.1007/s13205-020-2126-5.
Eleazu CO. Characterization of the natural products in cocoyam (Colo- casia esculenta) using GC-MS. Pharm Biol. 2016;54(12):2880–2885. https://doi.org/10.1080/13880209.2016.1190383.
Kolar MJ, Konduri S, Chang T, Wang H, Mcnerlin C, Ohlsson L. Linoleic acid esters of hydroxy linoleic acids are anti-inflammatory lipids found in plants and mammals. J Biol Chem. 2019;294(27):10698–707. https://doi.org/10.1074/jbc.ra118.006956.
Perona JS, Arcemis C, Ruiz-Gutierrez V, Catalá A. Effect of dietary high-oleic-acid oils that are rich in antioxidants on microsomal lipid peroxidation in rats. J Agric Food. 2005;53(3):730–735. https://doi.org/10.1021/jf048374p.
Abdollahi A, Fasihi-Ramandi M, Kouhpayeh S, Najafipour S, Meshkibaf M, Naghdi M. Antimicrobial Effect of 15 Medicinal Plant Species and their Dependency on Climatic Conditions of Growth in Different Geographical and Ecological Areas of Fars Province. ZJRMS. 2012;14:31–90. Available from: https://www.researchgate.net/publication/277230263_Antimicrobial_Effect_of_ 15_Medicinal_Plant_Species_and_their_Dependency_on_Climatic_Conditions_ of_Growth_in_Different_Geographical_and_Ecological_Areas_of_Fars_Province.
Mpinda C, Meyer D, Lyantagaye S. In vitro antimicrobial activity of extracts of some plant species used in the management of HIV/AIDS in Tanzania. Tanzania Journal of Science. 2018;44(1):179–90. Available from: https://www.ajol.info/index.php/tjs/ article/view/171562.
Wang A, Wu H, Zhu X, Lin J. Species Identification of Conyza bonarien- sis Assisted by Chloroplast Genome Sequencing. Front Genet. 2018;9(374). https://doi.org/10.3389/fgene.2018.00374.
Berman J, K DJ. Drug resistance and tolerance in fungi 2020. Nat Rev Microbiol. 2020;18(6):319–331. https://doi.org/10.1038/s41579-019-0322-2.
Fuerst JA. Microorganisms-A Journal and a Unifying Concept for the Science of Microbiology. Microorganisms. 2014;2(4):140–146. https://doi.org/10.3390/microorganisms2040140.
Thabit RAS, Cheng XR, Tang X, Sun J, Shi YH, Le GW. Antioxidant and antibacterial activities of extracts from Conyza bonariensis growing in Yemen. Pak J Pharm Sci. 2015;28(1):129–163. 25553691. Available from: https://pubmed.ncbi.nlm.nih.gov/ 25553691/.
Gonelimali FD, Lin J, Miao W, Xuan J, Charles F, Chen M. Antimicro- bial Properties and Mechanism of Action of Some Plant Extracts Against Food Pathogens and Spoilage Microorganisms. Frontiers in Microbiology. 2018;9(1639). https://doi.org/10.3389/fmicb.2018.01639.
Girma Y, Jiru TM. Evaluation of Antimicrobial Activity of Conyza bonariensis Leaf Extracts against Clinically Isolated Fungi Causing Superficial Infection. Journal of Chemistry. 2021;https://doi.org/10.1155/2021/6367449.
Bipul B, Kimberly R, Fredrick MCL, Dwayne D, Anand Y. Antimicro- bial Activities of Leaf Extracts of Guava (Psidium guajava L.) on Two Gram- Negative and Gram-Positive Bacteria. Int J Microbiol. 2013;p. 746165–746165. https://doi.org/10.1155/2013/746165.
Karleigh B, Renee B, Cynthia D, Sean O, Andrew N, W R. Evaluation of different solvents to extract antibacterial compounds from jalapeño peppers. PMC. 2017;5(3). Available from: https://www.ncbi.nlm.nih.gov/pmc/articles/ PMC5448369/. https://doi.org/10.1002/fsn3.423.
Wang H, Kolar MJ, Chang T, Rizo J, Konduri S, Mcnerlin C, et al. Stereochemistry of Linoleic Acid Esters of Hydroxy Linoleic Acids. NIH. 2019;21(19):8080–8084. https://doi.org/10.1021/acs.orglett.9b03054.
Jarmo K, Holopainen VV, Rajendra P, Ghimire. Climate Change Effects on Sec- ondary Compounds of Forest Trees in the Northern Hemisphere | Plant Science. Frontiers . 2018;9(1445). Available from: https://www.frontiersin.org/articles/10. 3389/fpls.2018.01445/full. https://doi.org/10.3389/fpls.2018.01445.
Viszwapriya D, Prithika U, Deebika S, Balamurugan K, Pandian SK. In vitro and in vivo antibiofilm potential of 2,4-Di-tert-butylphenol from seaweed surface asso- ciated bacterium Bacillus subtilis against group A streptococcus. Microbiol Res. 2016;191:19–31. https://doi.org/10.1016/j.micres.2016.05.010.
Yang D, Michel L, Chaumont JP, Millet-Clerc J. Use of caryophyllene oxide as an anti- fungal agent in an in vitro experimental model of onychomycosis. Mycopathologia. 1999;148(2):79–82. https://doi.org/10.1023/a:1007178924408.
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