Protective Impact of Scutellaria pinnatifida Extract on Glyphosate-Induced Toxicity in Albino Rats
DOI:
https://doi.org/10.47419/bjbabs.v7i3.466Keywords:
Glyphosate, Scutellaria, malondyaldehyde, creatinine, low density lipoproteinAbstract
As herbicides become increasingly common for managing grass species, the popularity of medicinal plants, particularly those within the Lamiaceae family, is increasing due to their effectiveness and lower side effects. The genus Scutellaria is of particular interest. This study focuses on the administration of ascending doses of Scutellaria pinnatifida and its effect on glyphosate toxicity in albino female rats. A total of 32 rats were classified into four distinct groups. Group I served as the control and was provided a standard diet and water; Group II was provided a standard diet, water, and glyphosate; Group III was provided a standard diet, water, glyphosate, and S. pinnatifida extract; and Group IV was provided S. pinnatifida extract only. The administration was done daily for a fortnight. The findings showed a significant reduction in food consumption in the glyphosate group (P < 0.05) and an increase in kidney weight and white blood cell (WBC) counts. The liver, heart, and spleen weights increased non-significantly When compare control group, except spleen weight, which increased Spleen weight rise significant in Glyphosate and Scutellaria pinnatifida group compared to the control group. Serum creatinine levels showed a significant increase in the S. pinnatifida group, while low-density lipoprotein (LDL) levels were significantly elevated in the glyphosate-treated group. No statistically significant changes were observed in alanine aminotransferase (ALT), alkaline phosphatase (ALP), total cholesterol, triglycerides, or malondialdehyde (MDA) levels. Coadministration of S. pinnatifida extract partially ameliorated glyphosate-induced alterations, particularly in food intake, WBC count, and kidney weight. These findings suggest a moderate protective role of S. pinnatifida extract against glyphosate-induced physiological alterations.
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[1] Irvin L, Jackson C, Hill AL, Bajaj R, Mahmoudi C, Vaidya BN, Joshee N. Skullcaps (Scutellaria spp.): ethnobotany and current research. Medicinal Plants: From Farm to Pharmacy 2019;2019:141–168. https://doi.org/10.1007/978-3-030-31269-5_7
[2] Schlecht NJ, Lanier ER, Andersen TB, Brose J, Holmes D, Hamberger BR. CYP76BK1 orthologs catalyze furan and lactone ring formation in clerodane diterpenoids across the mint family. The Plant Journal 2024;120(3):984–997. https://doi.org/10.1111/tpj.17031
[3] Ganapathy AA, Priya VH, Kumaran A. Medicinal plants as a potential source of Phosphodiesterase-5 inhibitors: a review. Journal of Ethnopharmacology 2021;267:113536. https://doi.org/10.1016/j.jep.2020.113536
[4] Kim S-Y, Roy VC, Park J-S, Chun B-S. Extraction and characterization of bioactive compounds from brown seaweed (Undaria pinnatifida) sporophyll using two sequential green extraction techniques. Algal Research 2024;77:103330. https://doi.org/10.1016/j.algal.2023.103330
[5] Panzacchi S, Tibaldi E, De Angelis L, Falcioni L, Giovannini R, Gnudi F, et al. Carcinogenic effects of long-term exposure from prenatal life to glyphosate and glyphosate-based herbicides in Sprague–Dawley rats. Environmental Health. 2025;24(1):36.
[6] El-Shenawy NS. Oxidative stress responses of rats exposed to roundup and its active ingredient glyphosate. Environmental Toxicology and Pharmacology 2009;28(3):379–385. https://doi.org/10.1016/j.etap.2009.06.001
[7] Deba Z, Jambale TA, Kalasker PS, Jaweed SA. Study of assessment of change in lipid profile pattern in patients on hemodialysis. Journal of the Indian Medical Association 2024;122(7):55–57.
[8] Serra L, Estienne A, Vasseur C, Froment P, Dupont J. Mechanisms of glyphosate and glyphosate-based herbicides action in female and male fertility in humans and animal models. Cells 2021;10(11):3079. https://doi.org/10.3390/cells10113079
[9] Tang J, Hu P, Li Y, Win-Shwe T-T, Li C. Ion imbalance is involved in the mechanisms of liver oxidative damage in rats exposed to glyphosate. Frontiers in Physiology 2017;8:1083. https://doi.org/10.3389/fphys.2017.01083
[10] Boozari M, Mohammadi A, Asili J, Emami SA, Tayarani-Najaran Z. Growth inhibition and apoptosis induction by Scutellaria pinnatifida A. Ham. on HL-60 and K562 leukemic cell lines. Environmental Toxicology and Pharmacology 2015;39(1):307–312. https://doi.org/10.1016/j.etap.2014.12.002
[11] Sato T, Kameyama T, Ohori T, Matsuki A, Inoue H. Effects of eicosapentaenoic acid treatment on epicardial and abdominal visceral adipose tissue volumes in patients with coronary artery disease. Journal of Atherosclerosis and Thrombosis 2014;21(10):1031–1043. https://doi.org/10.5551/jat.23390
[12] Sammons RD, You J, Qi Y, Flasinski S, Kavanaugh C, Washam J, et al. Evaluation of glyphosate resistance in Arabidopsis thaliana expressing an altered target site EPSPS. Pest Management Science 2018;74(5):1174–1183. https://doi.org/10.1002/ps.4654
[13] Panzacchi S, Mandrioli D, Manservisi F, Bua L, Falcioni L, Spinaci M, et al. The Ramazzini Institute 13-week study on glyphosate-based herbicides at human-equivalent dose in Sprague-Dawley rats: study design and first in-life endpoints evaluation. Environmental Health 2018; 17:1–13. https://doi.org/10.1186/s12940-018-0393-y
[14] Pandey A, Rudraiah M. Analysis of endocrine disruption effect of Roundup® in adrenal gland of male rats. Toxicology Reports 2015;2:1075–1085. https://doi.org/10.1016/j.toxrep.2015.07.021
[15] Mungamuri SK, Javvadi Y. Role of dietary supplementation of natural products in the prevention and treatment of liver diseases. Phytochemicals Targeting Tumor Microenvironment in Gastrointestinal Cancers 2020;2020:261–285. https://doi.org/10.1007/978-3-030-48405-7_12
[16] Kang Y-M, Kim Y-J, Kim K. Significance of traditional herbal medicine for dyslipidemia. American Journal of Translational Research 2023;15(8):5373.
[17] Dedeke GA, Owagboriaye FO, Ademolu KO, Olujimi OO, Aladesida AA. Comparative assessment on mechanism underlying renal toxicity of commercial formulation of roundup herbicide and glyphosate alone in male albino rat. International Journal of Toxicology 2018;37(4):285–295. https://doi.org/10.1177/1091581818779553
[18] Talyn B, Muller K, Mercado C, Gonzalez B, Bartels K. The herbicide glyphosate and its formulations impact animal behavior across taxa. Agrochemicals 2023;2(3):367–408. https://doi.org/10.3390/agrochemicals2030022
[19] Samsel A, Seneff S. Glyphosate, pathways to modern dis-eases III: manganese, neurological diseases, and asso-ciated pathologies. Surgical Neurology International 2015;6:45. https://doi.org/10.4103/2152-7806.153876
[20] Catelan TBS, Radai JAS, Leitao MM, Branquinho LS, de Paula Vasconcelos PC, Heredia-Vieira SC, et al. Evaluation of the toxicity and anti-inflammatory activities of the infusion of leaves of Campomanesia guazumifolia (Cambess.) O. Berg. Journal of Ethnopharmacology 2018;226:132–142. https://doi.org/10.1016/j.jep.2018.08.015
[21] Jasper R, Locatelli GO, Pilati C, Locatelli C. Evaluation of biochemical, hematological and oxidative parameters in mice exposed to the herbicide glyphosate-Roundup®. Interdisciplinary Toxicology 2012;5(3):133–140. https://doi.org/10.2478/v10102-012-0022-5
[22] Aitte SA, Zain MH. Study of changes in blood parameters and calculation of PCT, MPV and DPW for the platelets of laboratory females and males of albino mice during exposure to doses of pyrethroid pesticide (Alphacypermethrin). IOSR Journal of Pharmacy and Biological Sciences 2019;14(2):71–78.
[23] Martins-Gomes C, Silva TL, Andreani T, Silva AM. Glyphosate vs. glyphosate-based herbicides exposure: a review on their toxicity. Journal of Xenobiotics 2022;12(1):21–40. https://doi.org/10.3390/jox12010003
[24] Parven A, Meftaul IM, Venkateswarlu K, Megharaj M. Herbicides in modern sustainable agriculture: environmental fate, ecological implications, and human health concerns. International Journal of Environmental Science and Technology 2024;1–22. https://doi.org/10.1007/ s13762-024-05818-y
[25] Mesnage R, Benbrook C, Antoniou MN. Insight into the confusion over surfactant co-formulants in glyphosate-based herbicides. Food and Chemical Toxicology 2019;128: 137–145. https://doi.org/10.1016/j.fct.2019.03.053
[26] Bradberry SM, Proudfoot AT, Vale JA. Glyphosate poisoning. Toxicological Reviews 2004;23(3):159–167. https://doi.org/10.2165/00139709-20042303000003
[27] Suk K. Regulation of neuroinflammation by herbal medicine and its implications for neurodegenerative diseases. Neurosignals 2005;14(1–2):23–33. https://doi.org/10.1159/000085383
[28] Turkmen R, Birdane YO, Demirel HH, Kabu M, Ince S. Protective effects of resveratrol on biomarkers of oxidative stress, biochemical and histopathological changes induced by sub-chronic oral glyphosate-based herbicide in rats. Toxicology Research 2019;8(2):238–245. https://doi.org/10.1039/C8TX00287H
[29] Rebai O, Belkhir M, Boujelben A, Fattouch S, Amri M. Morus alba leaf extract mediates neuroprotection against glyphosate-induced toxicity and biochemical alterations in the brain. Environmental Science and Pollution Research 2017;24(10):9605–9613. https://doi.org/10.1007/s11356-017-8584-6
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Copyright (c) 2026 Nazar M. Shareef Mahmood, Shorish Mustafa Abdullah Gorony, Ranjbar Muksy Mohammed Shareef, Kurdo Bapir Chato, Mahnaz Nooruldeen Yousif, Awring Ibrahim Mhamadamin
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